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Dependant upon the chemical structure with the analyte, the molecules are retarded within the column stationary stage as a consequence of precise intermolecular interactions involving the analyte as well as the packing product to the column.The peak retention volume is equivalent for the retention time with the analyte multiplied by movement level;
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Stationary phase chemistry dictates the affinity with the sample components to stay or retain over the column as being the cell phase moves the sample in the column. Therefore, the sample parts traverse the column and elute at different prices.Silica columns are widely used in the pharmaceutical analysis. The chromatography column packing by which
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